Separation of DNA by length in rotational flow: Lattice-Boltzmann-based simulations

Document Type

Article

Publication Title

Biomicrofluidics

Publication Date

7-1-2015

Abstract

We use a lattice-Boltzmann based Brownian dynamics simulation to investigate the separation of different lengths of DNA through the combination of a trapping force and the microflow created by counter-rotating vortices. We can separate most long DNA molecules from shorter chains that have lengths differing by as little as 30%. The sensitivity of this technique is determined by the flow rate, size of the trapping region, and the trapping strength. We expect that this technique can be used in microfluidic devices to separate long DNA fragments that result from techniques such as restriction enzyme digests of genomic DNA.

Volume

9

Issue

4

DOI

10.1063/1.4926667

ISSN

19321058

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