A comparison of two pH-stat carbon dioxide dosing systems for ocean acidification experiments
Document Type
Article
Publication Title
Limnology and Oceanography: Methods
Publication Date
9-1-2013
Abstract
As the oceans acidify due to increasing atmospheric CO2, there is a growing need to understand the impact of this process on marine organisms. Field observations are difficult because of multiple covarying factors (e.g., temperature, salinity). As such, there is interest in conducting controlled, laboratory experiments to best understand how changes in acidity will affect marine organisms. We tested two intermittent CO2 dosing systems, a "home aquarium hobby" grade pH controller and an industrial process control platform. We assessed stability, accuracy, and precision over 7-d experimental periods as well as relative cost of the two configurations. We also compared three laboratory-grade pH electrodes to the hobbyist electrode to further evaluate electrode quality on systemcontrolled pH stability and drift. Whereas the industrial system offered some benefit with regard to autonomy, our results show that the low-cost hobbyist system can be modified appropriately to provide comparable pH control. We provide a detailed list of procedures and software developed for the implementation of a cost-effective, precision-controlled CO2 dosing system to support laboratory-based ocean acidification experiments.
Volume
11
Issue
9
First Page
485
Last Page
494
DOI
10.4319/lom.2013.11.485
Recommended Citation
Wilcox-Freeburg, E., Rhyne, A., Robinson, W., Tlusty, M., Bourque, B., & Hannigan, R. (2013). A comparison of two pH-stat carbon dioxide dosing systems for ocean acidification experiments. Limnology and Oceanography: Methods, 11 (9), 485-494. https://doi.org/10.4319/lom.2013.11.485
E-ISSN
15415856