Investigating the Biosynthetic Potential of Pseudoalteromonas rubra Strain CH007
Education Level
Undergraduate
Faculty Advisor(s)
Professor Koty Sharpe
Academic Department(s)
Biomedical and Pharmaceutical Sciences, University of Rhode Island and Biology, Roger Williams University
Symposium Date
2024
Abstract
Crustose coralline algae (CCA) are a group of calcifying red algae that grow on coral reefs worldwide and have been shown to induce coral larval settlement. The marine bacterium Pseudoalteromonas rubra has been repeatedly isolated from CCA and may promote induction of coral larval settlement due to its production of specialized metabolites.
In this study, compounds produced from Pseudoalteromonas rubra strain CH007, isolated from CCA growing in the culture tanks at Roger Williams University, were purified using multiple chromatographic methods. The resulting fractions were investigated using high performance liquid-chromatography coupled with tandem mass-spectrometry (HPLC-MS/MS). CH007 was found to produce a series of pigmented molecules known as the prodiginines. Bioactivity testing of fractions enriched in prodiginines revealed antimicrobial properties against the fish pathogen Vibrio anguillarum strain M935m and the causative agent for Juvenile Oyster Disease, Roseovarius crassostreae.
Recommended Citation
Derreza, Emily; Hill, Margaret; Rowley, David; Sharp, Koty H.; and Hughes, Casidhe, "Investigating the Biosynthetic Potential of Pseudoalteromonas rubra Strain CH007" (2024). Student Research Symposium. 29.
https://docs.rwu.edu/studentresearchsymposium/29
Comments
This research was presented at the 2024 Rhode Island Summer Undergraduate Research Symposium, held on Friday, July 26, at the University of Rhode Island and supported by RI-INBRE.